{"claims": [{"text": "At P7 to P10, 95.7 percent of astrocytes responded to DHPG", "quote_or_locator": "Results section, age-dependent decline subsection: 'In the P07–P10 groups, 95.7% of the astrocytes (n = 118) responded to DHPG.'"}, {"text": "By P19 to P26, only 68.1 percent of astrocytes responded to DHPG", "quote_or_locator": "Results section, age-dependent decline subsection: 'and 68.1% in P19–P26 (n = 94)'"}, {"text": "Youngest astrocytes produced DHPG responses averaging 123 percent delta F", "quote_or_locator": "Results section, age-dependent decline subsection: 'The youngest group (P07–P10) presented the largest amplitudes, averaging 123.0 ± 5.2% ΔF (n = 113)'"}, {"text": "By P11 to P14, DHPG response amplitude dropped to 53 percent delta F", "quote_or_locator": "Results section, age-dependent decline subsection: 'whereas the older groups presented progressively lower mean amplitudes (P11–P14: 53.3 ± 3.7% ΔF, n = 93)'"}, {"text": "In the youngest group, 79.5 percent of astrocytes responded to adenosine", "quote_or_locator": "Results section, age-dependent decline subsection: 'In the youngest group (P07–P10), 79.5% of the astrocytes responded to adenosine (n = 263).'"}, {"text": "By P19 to P26, only 20 percent of astrocytes responded to adenosine", "quote_or_locator": "Results section, age-dependent decline subsection: 'and 20.0% at P19–P26 (n = 52)'"}, {"text": "Adenosine response amplitude in youngest group was 63.1 percent delta F", "quote_or_locator": "Results section, age-dependent decline subsection: 'In the P07–P10 groups, adenosine responses had a mean amplitude of 63.1 ± 3.1% ΔF (n = 210).'"}, {"text": "Adenosine response amplitude in oldest group was 24.3 percent delta F", "quote_or_locator": "Results section, age-dependent decline subsection: 'and 24.3 ± 4.7% ΔF from P19–P26 (n = 11)'"}, {"text": "DHPG-evoked calcium responses were mediated predominantly by mGluR5, not mGluR1", "quote_or_locator": "Results section, DHPG induced Ca2+ transients subsection: 'In contrast, the application of MTEP reduced the DHPG-induced Ca²⁺ transient to 15.2 ± 3.2% ΔF (n = 89), corresponding to 18% of the control amplitude, a highly significant reduction (Fig. 2E; p < 0.001)' and 'In the presence of JNJ 16259685, the amplitude of the DHPG-induced Ca²⁺ response reached 77.6 ± 6.2% ΔF (n = 87), corresponding to 71% of the first response, a difference that was not significantly different from the rundown control.'"}, {"text": "Adenosine-induced calcium signaling was mediated mainly by A2A receptors, not A2B", "quote_or_locator": "Results section, adenosine-induced Ca2+ signaling subsection: 'With respect to the cells that responded to adenosine, 75.3% (n = 77) also responded to the A2A receptor agonist PSB0777 (5 µM), whereas only 15.5% (n = 71) responded to the A₂B receptor agonist BAY 60-6583' and 'In the presence of ZM241385, adenosine-induced Ca²⁺ responses were significantly reduced to 34.9 ± 4.9% of the amplitude of the first response (control; n = 29; p < 0.001). In contrast, the A2B antagonist PSB603 (50 nM) did not significantly alter adenosine-evoked responses, which remained at 110.1 ± 12.9% of the first response (n = 20).'"}, {"text": "They analyzed 475 cells total across 16 brain slices from 12 animals in the DHPG rundown experiment", "quote_or_locator": "Results section, adenosine and DHPG induce Ca2+ transients subsection: 'Notably, 8% of the DHPG-responsive cells (38 out of 475 cells, 16 brain slices, 12 animals) did not respond to ADP'"}, {"text": "DHPG responses showed desensitization with repeated applications, dropping to 67.5 percent of first response on second application", "quote_or_locator": "Results section, adenosine and DHPG induce Ca2+ transients subsection: 'The first application of DHPG evoked a Ca²⁺ response with a mean amplitude of 82.8 ± 4.5% ΔF (n = 75). The second application produced a response that reached 86.4% of the initial amplitude and was significantly reduced (67.5 ± 3.3% ΔF; n = 75; p < 0.001).'"}, {"text": "Adenosine responses showed desensitization by the third application, dropping to 68.7 percent of first response", "quote_or_locator": "Results section, adenosine and DHPG induce Ca2+ transients subsection: 'By the third application, the response significantly decreased to 68.7% of the first response (38.7 ± 4.4% ΔF; n = 40; p < 0.001)'"}], "prompt_version": "p1.0", "verdicts": [{"claim": "At P7 to P10, 95.7 percent of astrocytes responded to DHPG", "verdict": "supported", "evidence": "In the P07–P10 groups, 95.7% of the astrocytes (n = 118) responded to DHPG.", "note": null}, {"claim": "By P19 to P26, only 68.1 percent of astrocytes responded to DHPG", "verdict": "supported", "evidence": "and 68.1% in P19–P26 (n = 94)", "note": null}, {"claim": "Youngest astrocytes produced DHPG responses averaging 123 percent delta F", "verdict": "supported", "evidence": "The youngest group (P07–P10) presented the largest amplitudes, averaging 123.0 ± 5.2% ΔF (n = 113)", "note": null}, {"claim": "By P11 to P14, DHPG response amplitude dropped to 53 percent delta F", "verdict": "supported", "evidence": "whereas the older groups presented progressively lower mean amplitudes (P11–P14: 53.3 ± 3.7% ΔF, n = 93)", "note": null}, {"claim": "In the youngest group, 79.5 percent of astrocytes responded to adenosine", "verdict": "supported", "evidence": "In the youngest group (P07–P10), 79.5% of the astrocytes responded to adenosine (n = 263).", "note": null}, {"claim": "By P19 to P26, only 20 percent of astrocytes responded to adenosine", "verdict": "supported", "evidence": "and 20.0% at P19–P26 (n = 52)", "note": null}, {"claim": "Adenosine response amplitude in youngest group was 63.1 percent delta F", "verdict": "supported", "evidence": "In the P07–P10 groups, adenosine responses had a mean amplitude of 63.1 ± 3.1% ΔF (n = 210).", "note": null}, {"claim": "Adenosine response amplitude in oldest group was 24.3 percent delta F", "verdict": "supported", "evidence": "and 24.3 ± 4.7% ΔF from P19–P26 (n = 11)", "note": null}, {"claim": "DHPG-evoked calcium responses were mediated predominantly by mGluR5, not mGluR1", "verdict": "supported", "evidence": "In contrast, the application of MTEP reduced the DHPG-induced Ca²⁺ transient to 15.2 ± 3.2% ΔF (n = 89), corresponding to 18% of the control amplitude, a highly significant reduction (Fig. 2E; p < 0.001) and In the presence of JNJ 16259685, the amplitude of the DHPG-induced Ca²⁺ response reached 77.6 ± 6.2% ΔF (n = 87), corresponding to 71% of the first response, a difference that was not significantly different from the rundown control.", "note": null}, {"claim": "Adenosine-induced calcium signaling was mediated mainly by A2A receptors, not A2B", "verdict": "supported", "evidence": "With respect to the cells that responded to adenosine, 75.3% (n = 77) also responded to the A2A receptor agonist PSB0777 (5 µM), whereas only 15.5% (n = 71) responded to the A₂B receptor agonist BAY 60-6583 and In the presence of ZM241385, adenosine-induced Ca²⁺ responses were significantly reduced to 34.9 ± 4.9% of the amplitude of the first response (control; n = 29; p < 0.001). In contrast, the A2B antagonist PSB603 (50 nM) did not significantly alter adenosine-evoked responses, which remained at 110.1 ± 12.9% of the first response (n = 20).", "note": null}, {"claim": "They analyzed 475 cells total across 16 brain slices from 12 animals in the DHPG rundown experiment", "verdict": "supported", "evidence": "Notably, 8% of the DHPG-responsive cells (38 out of 475 cells, 16 brain slices, 12 animals) did not respond to ADP", "note": null}, {"claim": "DHPG responses showed desensitization with repeated applications, dropping to 67.5 percent of first response on second application", "verdict": "supported", "evidence": "The first application of DHPG evoked a Ca²⁺ response with a mean amplitude of 82.8 ± 4.5% ΔF (n = 75). The second application produced a response that reached 86.4% of the initial amplitude and was significantly reduced (67.5 ± 3.3% ΔF; n = 75; p < 0.001).", "note": null}, {"claim": "Adenosine responses showed desensitization by the third application, dropping to 68.7 percent of first response", "verdict": "supported", "evidence": "By the third application, the response significantly decreased to 68.7% of the first response (38.7 ± 4.4% ΔF; n = 40; p < 0.001)", "note": null}]}